101 addgene plasmid Search Results


human immunodeficiency virus hiv 1 tat sequence  (Addgene inc)
92
Addgene inc human immunodeficiency virus hiv 1 tat sequence
Human Immunodeficiency Virus Hiv 1 Tat Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/101+addgene+plasmid/pmc07483933-13-13-23?v=Addgene+inc
Average 92 stars, based on 1 article reviews
human immunodeficiency virus hiv 1 tat sequence - by Bioz Stars, 2026-08
92/100 stars
  Buy from Supplier
binary vector psb161  (Addgene inc)
93
Addgene inc binary vector psb161
Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the <t>pSB161</t> plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.
Binary Vector Psb161, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/101+addgene+plasmid/bio_rxiv__2025__09__10__675371-157-1-29?v=Addgene+inc
Average 93 stars, based on 1 article reviews
binary vector psb161 - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
nls gfp plasmid  (Addgene inc)
90
Addgene inc nls gfp plasmid
Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the <t>pSB161</t> plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.
Nls Gfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/101+addgene+plasmid/pmc03831989-238-1-6?v=Addgene+inc
Average 90 stars, based on 1 article reviews
nls gfp plasmid - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
accepted 11 july 2023 stem cell research 71 2023 103166 continued  (Addgene inc)
91
Addgene inc accepted 11 july 2023 stem cell research 71 2023 103166 continued
Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the <t>pSB161</t> plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.
Accepted 11 July 2023 Stem Cell Research 71 2023 103166 Continued, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/101+addgene+plasmid/pm37473460-13-23-81?v=Addgene+inc
Average 91 stars, based on 1 article reviews
accepted 11 july 2023 stem cell research 71 2023 103166 continued - by Bioz Stars, 2026-08
91/100 stars
  Buy from Supplier
v2ths 41638 tripz inducible lentiviral human zfc3h1 shrna plasmid horizon  (Addgene inc)
92
Addgene inc v2ths 41638 tripz inducible lentiviral human zfc3h1 shrna plasmid horizon
Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the <t>pSB161</t> plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.
V2ths 41638 Tripz Inducible Lentiviral Human Zfc3h1 Shrna Plasmid Horizon, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/101+addgene+plasmid/pm38964322-976-249-274?v=Addgene+inc
Average 92 stars, based on 1 article reviews
v2ths 41638 tripz inducible lentiviral human zfc3h1 shrna plasmid horizon - by Bioz Stars, 2026-08
92/100 stars
  Buy from Supplier
deposit 85226 ids 231305 2131339  (Addgene inc)
93
Addgene inc deposit 85226 ids 231305 2131339
Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the <t>pSB161</t> plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.
Deposit 85226 Ids 231305 2131339, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/101+addgene+plasmid/pm39919271-392-24-23?v=Addgene+inc
Average 93 stars, based on 1 article reviews
deposit 85226 ids 231305 2131339 - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
cmv promoter  (Addgene inc)
93
Addgene inc cmv promoter
Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the <t>pSB161</t> plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.
Cmv Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/101+addgene+plasmid/bio_rxiv__64898__2025__12__22__696043-151-30-32?v=Addgene+inc
Average 93 stars, based on 1 article reviews
cmv promoter - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
vps4 hcp1  (Addgene inc)
90
Addgene inc vps4 hcp1
Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the <t>pSB161</t> plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.
Vps4 Hcp1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/101+addgene+plasmid/pmc06602582-15-3-8?v=Addgene+inc
Average 90 stars, based on 1 article reviews
vps4 hcp1 - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
pet19b backbone  (Addgene inc)
90
Addgene inc pet19b backbone
Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the <t>pSB161</t> plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.
Pet19b Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/101+addgene+plasmid/pmc06410776-127-22-30?v=Addgene+inc
Average 90 stars, based on 1 article reviews
pet19b backbone - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
pgex 4 t1  (Addgene inc)
92
Addgene inc pgex 4 t1
Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the <t>pSB161</t> plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.
Pgex 4 T1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/101+addgene+plasmid/pm37793472-301-10-24?v=Addgene+inc
Average 92 stars, based on 1 article reviews
pgex 4 t1 - by Bioz Stars, 2026-08
92/100 stars
  Buy from Supplier

Image Search Results


Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the pSB161 plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.

Journal: bioRxiv

Article Title: Systematic Optimization Enables Near-Perfect In Vitro Transformation Efficiencies for Spirodela polyrhiza (Greater Duckweed)

doi: 10.1101/2025.09.10.675371

Figure Lengend Snippet: Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the pSB161 plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.

Article Snippet: The binary vector pSB161 (pL2_pSB90_2×35S::GUS::tMAS), which harbors a GUS reporter gene with introns under the control of a double 35S promoter, was obtained from Erin Cram and Carolyn Lee-Parsons (Addgene plasmid #123197; http://n2t.net/addgene:123197 ; RRID:Addgene_123197).

Techniques: Transformation Assay, Cell Culture, Staining, Plasmid Preparation, Gene Expression